TEST OF THE ANTIFUNGICAL ACTIVITY OF CELERY LEAF EXTRACT (Apium graveolens L) ON THE GROWTH OF Pityrosporum ovale USING THE DISC DIFFUSION METHOD
Pityrosporum ovale is a microorganism that causes infectious diseases. To overcome the problem of infection, you can use natural ingredients containing picretin, berberine and palmatin compounds which are included in the alkaloid group compounds, flavonoids, saponins and tannins, one of which is found in celery leaves (Apium graviolens L). This study aims to determine the activity of 70% chlorosome and ethanol extracts on the inhibitory power of Pityrosporum ovale growth, determine the effect of celery leaf extract concentration on Pityrosporum ovale, and find out the differences in antifungal activity with the use of various tracers to Pityrosporum ovale. The extract obtained using the maceration method, was carried out by maceration for 3x24 hours with chlorosomes and 70% ethanol. The maserat results were obtained with two tracers then evaporated and perfected using waterbath until a thick extract of celery leaves was obtained. The antifungi activity test was carried out using Potato Dextro Agar media and the disc diffusion method was made with a concentration of 15%, 25% and 35% of the celery leaf extract of each distributor. The results of the study proved the antifungi activity of celery leaf extract against Pityrosporum ovale in each of the tracers, namely ethanol coating 15%, 25% and 35% respectively by 6,5 mm; 13 mm and 15,5 mm. While in chloroform coating 15% .25% and 35% respectively by 9 mm; 11 mm and 13,5 mm. Positive control of Ketomed Shampoo on ethanol solvent by 17,5 mm and on chlorosome solvent by 17 mm. Negative control with a 10% DMSO solvent does not indicate the presence of antifungal activity. From the results of this study, it was concluded that the concentration of 35% in both breeders had the highest antifungi activity in inhibiting the growth of Pityrosporum ovale.
Keywords: Antifungal, Celery Leaf, Disc Diffusion Method, Inhibition, Pityrosporum ovale