ANTIBACTERIAL ACTIVITY TEST OF AWAR-AWAR LEAF EXTRACT (Ficus septica Burm. F) WITH n-HEXANE AND WATER FILTERS AGAINST THE GROWTH OF Staphylococcus aureus BACTERIA

Abstract

Staphylococcus aureus is one of the bacteria that causes infectious diseases. This infectious disease is a problem that often occurs in developing countries, one of which is Indonesia with a prevalence value ranging from 20-80%. Infectious diseases, especially skin diseases, can usually progress from mild to serious infections, especially if Staphylococcus aureus is already present. This study aims to determine the antibacterial activity of leaf extract of awar-awar (Ficus septica Burm f) with n-hexane and water as a solvent. The research was conducted by extracting awar-awar leaves with n-hexane and water so that after being evaporated, a thick extract was obtained. The extract obtained was then tested for its antibacterial activity using nutrient agar media in the well diffusion method. The results obtained showed the antibacterial activity of each filter, namely n-hexane with a concentration of 10% having an inhibition zone diameter of 5.3 mm, 25% having an inhibition zone diameter of 10.6 mm, and 35% having an inhibition zone diameter of 15. 3 mm. While the water filter with a concentration of 10% has an inhibition zone diameter of 5 mm, 25% has an inhibition zone diameter of 8 mm and 35% has an inhibition zone diameter of 11.3 mm. The positive control of amoxicillin with a concentration of 30µg/50µL had an inhibition zone of 20 mm and the negative control, DMSO 10%, did not show any antibacterial activity. From the results of the study, it was concluded that the concentration of 35% in both extracts had the highest antibacterial activity in inhibiting the growth activity of Staphylococcus aureus bacteria

Keywords: antibacterial test, Ficus septica, Staphylococcus aureus, bacterial inhibition, well diffusion method